For reference, below are the types of xylene substitutes by their common brand names. However, the compound is unstable owing to its double bounds. 1 Product Result | Match Criteria: Product Name Synonym ... Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing; Sigma-Aldrich pricing. Clearant (Xylene substitute, Non-toxic, Low odor), [Gallon] Innovative Products to Support Lean ... Products to Support Environmental Green Initiatives Eliminate Xylene & Phosphates from your tissue processing Supporting Lean Laboratory Processes NO COMMOTION IN THE MOTION! Histo-Clear enhances the clarity and vibrance of acidophilic stains and improves staining of Harris' Hematoxylin with a brighter Eosin background. However, they vary greatly in efficacy. Hydrate the Section. Product documentation Furthermore its low risk of igniting makes it safer to work with. Xylene Substitute is a flammable product and must be stored in a flammable fire cabinet. Clear-Advantage is a specific fraction of petrochemical derivatives that creates a safer xylene substitute for processing, staining and coverslipping. Research conducted by CBG Founder and Chairman, Gerald W. Camiener, Ph.D., demonstrated that the toxic aspects of aromatic solvents are associated with the double bonds present in their benzene rings—and that performance and chemical activities are associated with only the ring structure in the molecules. Xylene, an aromatic hydrocarbon is the most routinely used clearing agent during tissue processing but it is considered to be hazardous. chemical waste image by Antonio Oquias from Fotolia.com. The paraffin is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes.… CBG Biotech provides solvent distillation systems for industrial, laboratory and cannabis applications. To offset the additional cost (and then some), recycling xylene and other aromatic solvent substitutes can help your lab save substantially on solvent spend, while also exponentially reducing your lab’s waste disposal costs—by up to 50 times, depending on how much solvent waste your lab generates. In terms of performance, the d-limonenes are the next best type of xylene substitute. Neo-Clear® Xylene Substitute CAS 8052-41-3 Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing - Find MSDS or SDS, a COA, data sheets and more information. It also has a high vapour pressure (4.5mm Hg at 25oC) and has a penetrating odour (Hans et al., 1995). But only one option is as good as or better than xylene itself. In routine applications, the broad use of xylene has determined the standard of results by which all other products are measured. These safe alternatives are gentle toward tissue and do not cause shrinkage or morphology changes. The National Institute for Occupational Safety and Health (NIOSH) puts xylene’s Immediately Dangerous to Life or Health (IDLH) air concentration value at 900 ppm, which is essentially the point at which a worker will experience almost immediate negative health effects. Air Quality; This research led to the development of naphthenic solvents that are similar in performance. Hematology labs that test blood rely on both of these chemicals as solvents, while hospital laboratories can use either chemical in preparing tissue … Product documentation Neo-Clear. References: 1. Although xylene is used widely as a clearing agent for tissue processing it is a toxic reagent and some laboratories prefer to use less-toxic alternatives such as isopropanol or other xylene substitutes. Clearene is a selected blend of terpenes formulated specifically to replace xylene and toluene in histology and cytology laboratory procedures. Image-iT Plant Tissue Clearing Reagent and Image-iT Plant Tissue Hard-Set Mountant are based on Visikol™ for Plant Biology™ and Visikol™ Mount. How Xylene Compares to Xylene Substitutes, Relative drying times at room temperature, Solvent Comparisons: < 1.0 = superior to xylene; > 1.0 = inferior to xylene. Twenty of the 30 substitutes for xylene on the market today are safer than using xylene itself. There are also substitutes for xylene on the market that are effectively compatible, which means they perform just as well if not better than xylene and are relatively easy to switch to (although, at CBG Biotech, we always suggest you implement this change one procedure at a time so that you’re able to verify results before completely switching over to a substitute). These reagents are the ones usually employed in "dry cleaning" and are less effective than xylene and cannot be used to dewax sections or clean the tissue processor. Neo-Clear can be used in tissue preparations to remove alcohol and other dehydrating agents from the tissue before embedding. Among various objections to using a substitute, some pathologists view xylene as being critical for proper tissue processing, staining and coverslipping (in part due to the paraffin infiltration, which renders the tissue section transparent), and lab managers may think substitutes are either too expensive and/or difficult to switch to. The poorest-performing substitutes for xylene are the paraffinic and isoparaffinic hydrocarbons and the mixtures of paraffinic hydrocarbons with glycol ethers. Product description: Xylene Substitute: Product name: Tissue-Tek Tissue Clear: Quantities ・1×10L ・1×5L: Support. If you don't allow cookies, you may not be able to use certain features of the web site including but not limited to: log in and see personalized content. …embedding system and is performed directly after the histoprocessing. Xylene Substitute. These different solvent types do have low toxicity and odor levels, but their effectiveness as solvents in applications is poor compared to xylene. The first step in performing an H&E stain is to dissolve all the wax away with xylene (a hydrocarbon solvent). Histo-Clear is a relatively new commercial clearing agent offered as a safe substitute for xylene. These different solvent types do have low toxicity and odor levels, but their effectiveness as solvents in applications is poor compared to xylene. Histo-Clear can directly substitute for xylene and yields excellent results in automated tissue processing. Common Negative Health Effects of Xylene*, • Skin, eye and respiratory tract irritation, • Motor incoordination and impairment of equilibrium, • Flushing, redness of the face and a sensation of increased body heat, • Increased salivation, tremors, dizziness and confusion, • Severe abdominal pains, nausea, vomiting and loss of consciousness (if ingested), • Menstrual disorders and increased occurrence of miscarriages and birth defects, • Reduced blood platelet counts and increased chance of leukemia. In reality, using and recycling xylene substitutes will actually reduce your lab’s solvent spend while promoting safety. These safe alternatives are gentle toward tissue and do not cause shrinkage or morphology changes. While naphthenic hydrocarbon solvents do cost a little more than the aromatic solvents they replace, the extra cost is insignificant when you consider how much safer they make labs for employees. Histosol is the original high flash point (114 degree F TCC) histological clearing agent. The majority of cell and tissue components have no natural color and are not visible. compare. A xylene substitute for use in histology and cytology. 4 The trade names of xylene are xylol, dimethyl benzene, mixed xylene and violet 3. Rapid Clearing without the Toxic Hazards of Xylene The paraffinic and isoparaffinic hydrocarbons and the mixtures of paraffinic hydrocarbons with glycol ethers components have no natural and. Mit Tissue-Tek ® Glas™ Tissue-Mount™ for glass coverslipping 4 per Case ) Qty Price: $ /! To xylene ensure your lab ’ s Formula 83™, or one or our aromatic. But it is safer and a more preferable alternative to xylene cell and tissue components have no natural and. A hydrocarbon solvent ) processing but it is considered to be used in processing. Systems for industrial, laboratory and cannabis applications seines niedrigen Entzündungsrisikos sicherer Superior for! 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